Composite

Part:BBa_K3827004:Design

Designed by: Livia Vieira   Group: iGEM21_British_Columbia   (2021-10-20)


pLactate - luxCDE - lldR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3881
    Illegal NheI site found at 3904
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1571
    Illegal SapI.rc site found at 2697


Design Notes

luxCDE sequence is taken from BioBrick BBa_K325909. They are taken together given they form a heterocomplex that functions as a fatty acid reductase (Brodl et al, 2018).

Source

pLactate and lldR (Salmonella enterica complete genome NCBI)

luxCDE sequence is taken from BioBrick BBa_K325909

References

Brodl, E., Winkler, A., & Macheroux, P. (2018). Molecular mechanisms of bacterial bioluminescence. Computational and structural biotechnology journal, 16, 551-564.